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Figure 1. RET activation with <t>GDNF/GFRa1</t> has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)
Gdnf Gfra1 Mixed, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gdnf+gfra1+mixed/Recombinant+Human+GDNF+Protein/pm37921725-63-7-14
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Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)

Journal: Cytotherapy

Article Title: Impact of the overexpression of the tyrosine kinase receptor RET in the hematopoietic potential of induced pluripotent stem cells (iPSCs).

doi: 10.1016/j.jcyt.2023.10.003

Figure Lengend Snippet: Figure 1. RET activation with GDNF/GFRa1 has no effect on hematopoietic potential at D13 of iPSCs hematopoietic differentiation. (A) Morphology of non-adherent, round, hematopoietic cells derived from PB33-WT iPSC at D13 of differentiation. (B) Microscope pictures (magnification 10x) of May-Grunwald and Giemsa (MGG) staining at day +13 of floating hematopoietic cells differentiated from PB33-WT. (C) Microscope picture of CFU assay at D+14 of the culture displaying a CFU-GM (white) and BFU-E (red) colony. (DG) Phenotypic analysis of hemato- poietic cells initially gated on live cells. Proportion of CD34 total (D), primitive hematopoietic stem and progenitor cells (CD34+/CD38) (E), HSC phenotype bearing cells (CD34+/CD38/ CD49f+) (F), or total hematopoietic cells (CD45+) (G) at day +13 of hematopoietic differentiation with or without GDNF for 2 different iPSC cell lines (PB33-WT and PB68-WT). (H) CFC assays derived from PB33-WT and PB68-WT with or without GDNF showing the number of colonies per 5000 cells (Means and SD are represented) and the type of colonies (I). All experiments have been performed 5 times. P-values were calculated using a 2-tailed Student’s t-test. ns, not significant. (Color version of figure is available online.)

Article Snippet: For the GDNF/GFRa1 experiment, 100 ng/mL of GDNF & GFRa1 mixed 1:1; (212-GD-010, 714-GR-100; R&D Systems, France) were added to the media.

Techniques: Activation Assay, Derivative Assay, Microscopy, Staining, Colony-forming Unit Assay